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Kefalov, PhD, associate professor of ophthalmology and visual sciences at Washington University. The findings are published Dec.

Collaborators include scientists in Cleveland, Poland, Switzerland and Norway,. The research was initiated after scientists on the research team reported seeing occasional flashes of green light while working with an infrared laser. Unlike the laser pointers used in lecture halls or as toys, the powerful infrared laser the scientists worked with emits light waves thought to be invisible to the human eye.

Vinberg, Kefalov and their colleagues examined the scientific literature and revisited reports of people seeing infrared light. They repeated previous experiments in which infrared light had been seen, and they analyzed such light from several lasers to see what they could learn about how and why it sometimes is visible.

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Ultraviolet Spectroscopy and UV Lasers

Normally, a particle of light, called a photon, is absorbed by the retina, which then creates a molecule called a photopigment, which begins the process of converting light into vision. In standard vision, each of a large number of photopigments absorbs a single photon. But packing a lot of photons in a short pulse of the rapidly pulsing laser light makes it possible for two photons to be absorbed at one time by a single photopigment, and the combined energy of the two light particles is enough to activate the pigment and allow the eye to see what normally is invisible.

That's how we are able to see it.

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Although the researchers are the first to report that the eye can sense light through this mechanism, the idea of using less powerful laser light to make things visible isn't new. The two-photon microscope, for example, uses lasers to detect fluorescent molecules deep in tissues.


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And the researchers said they already are working on ways to use the two-photon approach in a new type of ophthalmoscope, which is a tool that allows physicians to examine the inside of the eye. The idea is that by shining a pulsing, infrared laser into the eye, doctors might be able to stimulate parts of the retina to learn more about its structure and function in healthy eyes and in people with retinal diseases such as macular degeneration.

UV Vis spectroscopy

The research was made possible, in part, by the Kefalov team's development of a tool that allowed the scientists to obtain light responses from retinal cells and photopigment molecules. That device already is commercially available and being used at several vision research centers around the world.

Energy states of real diatomic molecules

Materials provided by Washington University in St. Original written by Jim Dryden. Note: Content may be edited for style and length. Science News.

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Collaborators include scientists in Cleveland, Poland, Switzerland and Norway, The research was initiated after scientists on the research team reported seeing occasional flashes of green light while working with an infrared laser. Kefalov, Krzysztof Palczewski. Continuous wave operation.


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